Craftek Instruments provided a semi-automatic rotary Microtome for the successful training course on bone tissue surface decalcification at the China Medical Equipment Conference (CMEC) held in Suzhou, China from July 19th to 21st, hosted by the Department of Pathology of the First Affiliated Hospital of Fujian Medical University.
In the morning training session on July 19, Deputy Chief Technician Wu Ping from the Department of Pathology, The First Affiliated Hospital of Fujian Medical University taught “Application of Surface Decalcification Method in Bone Tissue Preparation”.
Unlike the traditional decalcification of bone tissue, which uses a strong acid soak to remove calcium salts and soften the bone tissue for sectioning, the new surface decalcification process does not decalcify the tissue prior to tissue fixation and dehydration embedding, and surface decalcification is performed at the side of the Microtome when tissue sectioning is used. The old decalcification method processed tissues with blurred HE staining and complete loss of antigenic and nucleic acid components, making accurate diagnosis and further molecular pathology testing impossible. The new decalcification method, however, ensures the vividness of HE staining and the preservation of antigenic and nucleic acid components of tissues, which is conducive to the smooth development of molecular pathology, as the decalcification time is controlled.
After the theoretical lecture, the instructors from the Department of Pathology, The First Affiliated Hospital of Fujian Medical University, led the participants to practice the surface decalcification of bone tissues on a Craftek Microtome.
During the two-day training, Shanshan Huang, Guoping Li, Pengcheng Wang, Hongyan Wu, and Saifan Zeng gave training lectures and hands-on practice in tissue production and dyeing.
We are grateful to Cody Instruments for providing the training course with a cold table, an integrated spreading and baking Microtome and a blower type Microtome dryer.
